I almost never see pixel art this good. Ash and May would make such a cute couple, though Misty might not like that idea very much. And Harley's pretty flamboyant in this one.
They looks a lot like you'd see them in the anime, but not quite...In the second image, for example, Ash's face is very slightly elongated. Also, May's legs are skinnier in the anime...though May indeed looks a great deal better in this one. She looks much older than 12 (or is she 10 in the show?), but then again, so does Ash.
(Of course, one rule of anime is that girls start puberty at age 5. Taken right from the Uncyclopedia.)